|Title||In vitro infection of human primary adipose cells with HIV-1: a reassessment.|
|Publication Type||Journal Article|
|Year of Publication||2003|
|Authors||Munier, S, Borjabad, A, Lemaire, M, Mariot, V, Hazan, U|
|Journal||AIDS (London, England)|
|Date Published||2003 Nov 21|
|Keywords||Adipocytes, Adipose Tissue, Antigens, CD4, Gene Expression, Hela Cells, HIV Infections, HIV-1, Humans, Receptors, CCR5, Receptors, CXCR4, Transfection|
We reassessed the infection ability of human primary preadipocytes. The use of X4, R5 or VSV-G-pseudotyped viral particles indicated that viral entry is the limiting step. However, transfection with HIV-1 receptors restored efficient infection. Analyses of CD4, CXCR4 and CCR5 expression on preadipocytes and adipocytes revealed that receptor co-expression levels did not permit HIV-1 entry into adipose cells from all biopsies tested. We concluded that adipose tissue cannot be infected with HIV-1 in vivo.