|Title||Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Okello, JBA, Rodriguez, L, Poinar, D, Bos, K, Okwi, AL, Bimenya, GS, Sewankambo, NK, Henry, KR, Kuch, M, Poinar, HN|
|Keywords||DNA, Complementary, HIV, Humans, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Reverse Transcription, RNA, Viral, RNA-Directed DNA Polymerase|
The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as possible, especially when dealing with degraded or low copy RNA samples. While there are many reverse transcriptases in the market, all claiming to be highly sensitive, there is need for a systematic independent comparison of their applicability in quantification of rare RNA transcripts or low copy RNA, such as those obtained from archival tissues.
|Alternate Journal||PLoS ONE|