Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA.

TitleQuantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA.
Publication TypeJournal Article
Year of Publication2010
AuthorsOkello, JBA, Rodriguez, L, Poinar, D, Bos, K, Okwi, AL, Bimenya, GS, Sewankambo, NK, Henry, KR, Kuch, M, Poinar, HN
JournalPloS one
Volume5
Issue11
Paginatione13931
Date Published2010
ISSN1932-6203
KeywordsDNA, Complementary, HIV, Humans, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Reverse Transcription, RNA, Viral, RNA-Directed DNA Polymerase
Abstract

The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as possible, especially when dealing with degraded or low copy RNA samples. While there are many reverse transcriptases in the market, all claiming to be highly sensitive, there is need for a systematic independent comparison of their applicability in quantification of rare RNA transcripts or low copy RNA, such as those obtained from archival tissues.

Alternate JournalPLoS ONE
Full Text
PubMed ID21085668
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