Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides.

TitleIdentification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides.
Publication TypeJournal Article
Year of Publication2008
AuthorsWayengera, M, Kajumbula, H, Byarugaba, W
JournalTheoretical biology & medical modelling
Volume5
Pagination18
Date Published2008
ISSN1742-4682
KeywordsAnti-Infective Agents, Biomedical Research, Deoxyribonucleases, Type II Site-Specific, DNA Restriction Enzymes, Genome, Viral, Herpesvirus 2, Human, HIV Infections, Humans, Peptide Fragments, Viral Proteins
Abstract

Herpes Simplex virus types 1 and 2 are enveloped viruses with a linear dsDNA genome of approximately 120-200 kb. Genital infection with HSV-2 has been denoted as a major risk factor for acquisition and transmission of HIV-1. Developing biomedical strategies for HSV-2 prevention is thus a central strategy in reducing global HIV-1 prevalence. This paper details the protocol for the isolation of restriction endunucleases (REases) with potent activity against the HSV-2 genome and models two biomedical interventions for preventing HSV-2.

Alternate JournalTheor Biol Med Model
Full Text
PubMed ID18687114
AttachmentSize
PDF icon Wayengera2008b-Microbiology-res.pdf2.83 KB