|Title||Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides.|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||Wayengera, M, Kajumbula, H, Byarugaba, W|
|Journal||Theoretical biology & medical modelling|
|Keywords||Anti-Infective Agents, Biomedical Research, Deoxyribonucleases, Type II Site-Specific, DNA Restriction Enzymes, Genome, Viral, Herpesvirus 2, Human, HIV Infections, Humans, Peptide Fragments, Viral Proteins|
Herpes Simplex virus types 1 and 2 are enveloped viruses with a linear dsDNA genome of approximately 120-200 kb. Genital infection with HSV-2 has been denoted as a major risk factor for acquisition and transmission of HIV-1. Developing biomedical strategies for HSV-2 prevention is thus a central strategy in reducing global HIV-1 prevalence. This paper details the protocol for the isolation of restriction endunucleases (REases) with potent activity against the HSV-2 genome and models two biomedical interventions for preventing HSV-2.
|Alternate Journal||Theor Biol Med Model|