Cross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library.

TitleCross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library.
Publication TypeJournal Article
Year of Publication2004
AuthorsKarle, S, Planque, S, Nishiyama, Y, Taguchi, H, Zhou, Y-X, Salas, M, Lake, D, Thiagarajan, P, Arnett, F, Hanson, CV, Paul, S
JournalAIDS (London, England)
Volume18
Issue2
Pagination329-31
Date Published2004 Jan 23
ISSN0269-9370
KeywordsDose-Response Relationship, Immunologic, Epitopes, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Immunoglobulin Fragments, Leukocytes, Mononuclear
Abstract

A single-chain fragment containing antibody V domains (scFv) isolated from a lupus antibody library displayed the ability to bind gp120 and the conserved gp120 determinant composed of residues 421-436. The scFv neutralized R5 and X4-dependent HIV-1 strains from clades B, C, and D. The lupus repertoire may be useful as a source of neutralizing antibodies to HIV.

Alternate JournalAIDS
Full Text
PubMed ID15075554